How to Test Ovulation in a Female Dog: A Scientific Method and a Practical Guide
In the field of pet breeding, accurately grasping the ovulation period of a female dog is the key to improving the success rate of conception. Whether you are a professional breeder or a family pet owner, it is crucial to understand the scientific methods of detecting ovulation. This article will combine the hot pet care topics on the Internet in the past 10 days to give you a detailed introduction to practical tips for detecting ovulation in female dogs.
1. Basic knowledge of female dog ovulation cycle

The estrus cycle of a female dog is usually divided into 4 phases, of which ovulation occurs in estrus (Estrus). Here is a typical periodic table:
| stage | duration | Main features |
|---|---|---|
| Early stage (Proestrus) | 7-10 days | Swelling of the vulva with bloody discharge |
| Estrus | 5-9 days | Receptive to mating, secretions become lighter |
| Later period (Diestrus) | About 60 days | Pregnancy or pseudopregnancy status |
| Anestrus | 3-4 months | Reproductive system resting period |
2. 5 scientific methods to detect ovulation
1.vaginal cytology
The ovulation period can be determined by observing changes in vaginal epithelial cells under a microscope. This method is commonly used by professional veterinarians, and the accuracy can reach more than 80%.
| cell type | Change in proportion | Corresponding stage |
|---|---|---|
| parabasal cells | >70% | proestrus |
| intermediate cells | 30-50% | Early estrus |
| keratinocytes | >80% | Ovulation period |
2.Progesterone level testing
Blood progesterone levels are the gold standard for determining ovulation. Here are the key figures:
| Progesterone value (ng/mL) | physiological significance |
|---|---|
| <2 | Not ovulating |
| 2-5 | 1-2 days before ovulation |
| 5-10 | Ovulating |
| >10 | 2-3 days after ovulation |
3.LH hormone test
Ovulation occurs 24-48 hours after the peak of luteinizing hormone (LH). Special test strips can be used to detect urine LH levels.
4.Ultrasound examination
By observing the development of follicles through B-ultrasound, you can visually see the changes in follicle size:
| follicle diameter | developmental stage |
|---|---|
| 3-5mm | early development |
| 6-7mm | close to maturity |
| 7mm | Ovulation is about to happen |
5.behavioral observation method
Judgment based on changes in the female dog’s behavior: standing reaction, tail deviation, active approach to the male dog, etc. are all important signals.
3. Practical testing suggestions
1.Testing time schedule
It is recommended to test every day starting from the estrus period, and different methods can be used in combination. The following are recommended testing frequencies:
| method | Recommended frequency |
|---|---|
| Progesterone test | every 1-2 days |
| vaginal cytology | every 2-3 days |
| B-ultrasound examination | every 3 days |
2.Things to note
• Ovulation times vary between dog breeds
• Ovulation may be irregular in older female dogs
• Environmental stress can affect hormone levels
• It is recommended to keep complete test records for veterinary reference
4. Frequently Asked Questions
Q: How can families easily determine ovulation?
A: You can observe changes in the degree of swelling of the vulva. When the swelling begins to subside and the secretion turns light yellow, it is usually close to the ovulation period.
Q: How much does the testing cost?
A: Progesterone testing costs about 200-300 yuan/time, B-ultrasound examination costs 150-400 yuan/time, and LH test strips cost about 50 yuan/piece.
Q: What should I do if I miss ovulation?
A: Female dogs usually only ovulate once per estrus cycle. If they miss it, they need to wait for the next estrus, which is about 6 months apart.
Mastering scientific ovulation detection methods can significantly improve the reproductive success rate. It is recommended that first-time breeders consult a professional veterinarian to develop a personalized testing plan. By accurately determining the time of ovulation, you can not only increase the pregnancy rate, but also avoid unnecessary matings and ensure the health of the female dog.
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